Analysis of the effectiveness of various protein coatings for optimizing the endothelialization of polymer matrices

Background . Due to the constant increase in number of surgeries restore blood flow affected vessels, development synthetic prostheses is relevant. One key success factors an adhesive properties inner surface, since rapid endothelialization vascular considered a factor necessary prevent thrombosis and neointimal hypertrophy. Aim : To determine effect surface modification polymer matrices with fibrin, fibronectin, or type I collagen on adhesion viability endothelial cells. Material Methods Polymer prepared by electrospinning from mixture poly(3-hydroxybutyrate-co-3-hydroxyvalerate) poly(ε-caprolactone) were studied. Matrix samples coated fibronectin fibrin. Then, human umbilical vein cells (HUVEC) colonized cultured for 3 days. Unmodified culture plate wells used as controls. Cell was assessed combined staining Hoechst 33342 ethidium bromide. The metabolic activity studied using MTT test. analyzed F-actin. Statistical analysis results performed GraphPrism 7.0 program. Results It found that adherent their did not differ unmodified ones. Coating demonstrated higher rates cell surface. However, rather high level death this group indicates such cannot fully ensure normal functioning Finally, we observed best when fibrin coating, which comparable plastic terms Conclusion Modification can significantly improve be small-diameter vessels.